Mitoplast
A mitoplast is a mitochondrion that has been stripped of its outer membrane leaving the inner membrane intact.[1]
In Electrophysiology
Diagram of mitoplast preparation for electrophysiological analysis. Remnants of the outer mitochondrial membrane remain. IMM = inner mitochondrial membrane (green), OMM = outer mitochondrial membrane (blue)
Mitoplasts are useful for electrophysiological analysis of mitochondrial function. Specifically, patch-clamp electrophysiology has emerged as a novel method for studying functionality of the inner mitochondrial membrane (IMM).[2] This method facilitates sensitive current measurement across the IMM, useful for studying the electron transport chain and proton leak (i.e., the uncoupling of proton movement down its electrochemical gradient and ATP synthesis via ATP synthase).
References
- "Mitoplast". Glossary of Biochemistry and Molecular Biology. GenScript.
- Bertholet, Ambre M.; Kirichok, Yuriy (2020). "Patch-Clamp Analysis of the Mitochondrial H+ Leak in Brown and Beige Fat". Frontiers in Physiology. 11: 326. doi:10.3389/fphys.2020.00326. ISSN 1664-042X. PMC 7174661. PMID 32351404.
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